Methods in recombinant protein production, 

Автор: Brigitte Gasser; Diethard Mattanovich
Название: Recombinant Protein Production in Yeast
ISBN: 1493990233 ISBN-13(EAN): 9781493990238
Издательство: Springer
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Автор: Moulton
Название: Fed-Batch Fementation: A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli
ISBN: 1907568921 ISBN-13(EAN): 9781907568923
Издательство: Elsevier Science
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Описание: Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.

Автор: Otto-Wilhelm Merten; D. Mattanovich; C. Lang; G. L
Название: Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology
ISBN: 9048157560 ISBN-13(EAN): 9789048157563
Издательство: Springer
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Описание: Selected Articles from the Meeting of the EFB Section on Microbial Physiology, Semmering/A, 5-8 October 2000

Название: Recombinant Protein Expression: Prokaryotic Hosts and Cell-Free Systems, 659
ISBN: 0323901468 ISBN-13(EAN): 9780323901468
Издательство: Elsevier Science
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Recombinant Protein Expression, Part A, Volume 659 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more.

Название: Recombinant Protein Expression: Eukaryotic Hosts, 660
ISBN: 0323907377 ISBN-13(EAN): 9780323907378
Издательство: Elsevier Science
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Recombinant Protein Expression, Part B, Volume 660 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more.

Автор: Murooka, Yoshikatsu
Название: Recombinant Microbes for Industrial and Agricultural Applications
ISBN: 082479141X ISBN-13(EAN): 9780824791414
Издательство: Taylor&Francis
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Автор: Venkatesh Balan
Название: Microbial Lipid Production
ISBN: 1493994867 ISBN-13(EAN): 9781493994861
Издательство: Springer
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Автор: Robert E. Rhoads
Название: Synthetic mRNA
ISBN: 1493936239 ISBN-13(EAN): 9781493936236
Издательство: Springer
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Описание: This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy.

Автор: S. Mohan Jain; S.K. Sopory; R.E. Veilleux
Название: In Vitro Haploid Production in Higher Plants
ISBN: 0792335775 ISBN-13(EAN): 9780792335771
Издательство: Springer
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Описание: This study covers historical and fundamental aspects of haploidy in crop improvement and deals with methods of haploid production, including anther culture, micropore culture, ovary culture, pollination with irradiated pollen, in vitro pollination and special culture techniques.

Автор: Shukla, Arun K.
Название: Integrated Methods In Protein Biochemistry: Part A,675
ISBN: 0323992668 ISBN-13(EAN): 9780323992664
Издательство: Elsevier Science
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Автор: Nikolaos E. Labrou
Название: Protein Downstream Processing
ISBN: 1627039767 ISBN-13(EAN): 9781627039765
Издательство: Springer
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Описание:

Part I. Screening and Design Purification Strategies

1. Protein Purification: An Overview

Nikolaos Labrou

2. Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE

Ignacio Amadeo, Laura Mauro, Eduardo Ortн, and Guillermina Forno

3. High Throughput Process Development: I. Process Chromatography

A. S. Rathore and R. Bhambure

4. High Throughput Process Development: II. Membrane Chromatography

A. S. Rathore and S. Muthukumar

5. Media Selection in Ion Exchange Chromatography in a Single Microplate

Charlotte Cabanne and Xavier Santarelli

6. High Throughput Screening of Dye-Ligands for Chromatography

Sunil Kumar and Narayan S. Punekar

7. Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips

Sunil Chhatre and Daniel G. Bracewell

Part II. Low-Resolution Protein Purification Methods

8. Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline

Zhengrong GU

9. Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from Plants

Oscar Aguilar and Marco Rito-Palomares

10. Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins

A. S. Rathore and R. Bhambure

11. Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP)

Ipsita Roy, Kalyani Mondal, and Munishwar N. Gupta

12. Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and Periplasm

Ario de Marco

13. Immunoglobulin Purification by Caprylic Acid

Victor Morais, Patricia Berasain, and Hugo Massaldi

Part III: High-Resolution Protein Purification Methods

14. Affinity Tags in Protein Purification and Peptide Enrichment: An Overview

Ana Sofia Pina, Нris L. Batalha, and Ana Cecнlia A. Roque

15. Expression and Purification of Large Active GST Fusion Enzymes

Stefania Deceglie, Claudia Lionetti, Marina Roberti, Palmiro Cantatore, and Paola Loguercio Polosa

16. Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion Proteins

Sharon Williams, Phil Morton, and Dev Baines

17. Zbasic - A Purification Tag for Selective Ion-Exchange Recovery

My Hedhammar, Johan Nilvebrant, and Sophia Hober

18. An Orthogonal Fusion Tag for Efficient Protein Purification

Johan Nilvebrant, Mikael Еstrand, and Sophia Hober

19. Phage Display of Engineered Binding Proteins

Mark Levisson, Ruud B. Spruijt, Ingrid Nolla Winkel, Servй W.M. Kengen, and John van der Oost

20. Biomimetic Affinity Ligands for Protein Purification

Isabel T. Sousa and M. Вngela Taipa

21. Synthesis and Application of Dye-Ligand Affinity Adsorbents

Evangelia Chronopoulou and Nikolaos E. Labrou

22. Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification

S. A. Camperi, M. C. Martнnez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio, and O. Cascone

23. Affinity Chromatography of Proteins on Monolithic Columns

E.G. Vlakh, G.A. Platonova, and T.B. Tennikova

24. Sample Displacement Batch Chromatography of Proteins

Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schlьter

Part IV. Assessing Protein Structural Integrity, Purity, and Stabilization

25. Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS Approaches

Catalin E. Doneanu, Weibin Chen, an

Автор: John M. Walker
Название: New Protein Techniques
ISBN: 0896031268 ISBN-13(EAN): 9780896031265
Издательство: Springer
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Описание: Inrecent years therehasbeen atremendousincreaseinour understandingofthe functioningofthe cellat the molecular leveL This has been achieved in the main by the invention and developmentof new methodology, particularlyin that area generally referred to as "genetic engineering. " Al- though this revolution has been taking place in the field of nucleic acids research, the protein chemist has at the same timedevelopedfresh methodologytokeeppace with the re- quirements ofpresent-daymolecularbiology. Today's mo- lecularbiologistscannolongerbecontentwithbeingexperts inoneparticulararea alone. Theyneedtobeequallycompe- tentin the laboratory at handling DNA, RNA, and proteins movingfrom one area toanotherasrequiredby theproblem thatisbeing solved. Althoughmanyof thenewtechniques in molecularbiologyare relativelyeasy tomaster, itisoften difficult for a researcher to obtain all the relevant informa- tionnecessaryforsettingupandsuccessfullyapplyinganew technique. Informationisofcourse availablein the research literature, but this often lacks the depth of description that the new user requires. This requirement for in-depth prac- tical details has become apparent by the considerable de- mand for places on our Molecular BiologyWorkshops held at Hatfield each summer. Volume 1of this series describedpracticalprocedures for a range of protein techniques frequently used by research workers in the field of molecular biology. Because of the limitations on length necessarily inherent in producing any v vi Preface book, one obviouslyhad to be selective in the choiceof titles forVolume1. TheproductionofVolume 3, therefore, allows the development of the theme initiated in Volume 1. This volumecontains afurther selection ofdetailed protocols for arangeofanalyticalandpreparativeproteintechniques, and should be seen as a continuation of Volume 1. Companion Volumes2and4provideprotocols fornucleic acid method- ology. Each methodisdescribedby an authorwhohas regularly used the technique in his or her ownlaboratory.