Описание: Selected Articles from the Meeting of the EFB Section on Microbial Physiology, Semmering/A, 5-8 October 2000
Название: Recombinant Protein Expression: Eukaryotic Hosts, 660 ISBN: 0323907377 ISBN-13(EAN): 9780323907378 Издательство: Elsevier Science Рейтинг: Цена: 26275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Recombinant Protein Expression, Part B, Volume 660 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more.
Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr(R) 250, and Expression of recombinant antibodies in Leishmania tarentolae.
Автор: Helmut K?nig; Harald Claus; Ajit Varma Название: Prokaryotic Cell Wall Compounds ISBN: 3642424821 ISBN-13(EAN): 9783642424823 Издательство: Springer Рейтинг: Цена: 25075.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Cell Wall Polymers and Structures of Bacteria.- The Murein Sacculus.- Occurrence, Structure, Chemistry, Genetics, Morphogenesis, and Functions of S-Layers.- Bacterial Polysaccharide Capsules.- Lipopolysaccharides.- Structure, Biosynthesis, and Function of Teichoic Acids and Related Cell Wall Glycopolymers in the Gram-positive Cell Envelope.- Outer Membrane Proteins.- Cell Wall Polymers and Structures of Archaea.- Cell Envelopes of Methanogens.- The Cell Envelopes of Haloarchaea: Staying in Shape in a World of Salt.- Cell Envelopes of Crenarchaeota and Nanoarchaeota.- Biological Activities.- Immunochemistry of the Cell Walls of Methanogenic Archaea: A View from the Past into the Future.- Cell Wall Structure and Pathogenicity.- Cell Wall Growth and Inhibition.- Cell Wall Targeted Antibiotics.- Bacterial Autolysins.- Cell Wall Interactions.- Prokaryotic Cell-Cell Interaction.- Adhesion of Bacteria to Protists.- Application of Cell Wall Components.- Prokaryotic Cell Wall Components: Structure and Biochemistry.- Accumulation of Heavy Metals by Micro-organisms: Biomineralization and Nanocluster Formation.
Автор: Byung Hong Kim, Geoffrey Michael Gadd Название: Prokaryotic Metabolism and Physiology ISBN: 1316622916 ISBN-13(EAN): 9781316622919 Издательство: Cambridge Academ Рейтинг: Цена: 9187.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Our biosphere is shaped by vast numbers of different prokaryotic microorganisms which show extremely diverse metabolic capabilities. This book provides an up-to-date overview of the metabolic processes that occur in bacteria and archaea, and the key processes that determine their roles in the environment, biotechnology and human health.
Автор: Byung Hong Kim, Geoffrey Michael Gadd Название: Prokaryotic Metabolism and Physiology ISBN: 1107171733 ISBN-13(EAN): 9781107171732 Издательство: Cambridge Academ Рейтинг: Цена: 25027.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Our biosphere is shaped by vast numbers of different prokaryotic microorganisms which show extremely diverse metabolic capabilities. This book provides an up-to-date overview of the metabolic processes that occur in bacteria and archaea, and the key processes that determine their roles in the environment, biotechnology and human health.
Автор: Peeters Название: Prokaryotic Gene Regulation ISBN: 1071624121 ISBN-13(EAN): 9781071624128 Издательство: Springer Рейтинг: Цена: 18904.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume presents a collection of versatile methodologies to investigate prokaryotic gene regulation, with focus on the different levels of information processing and usefulness for various model organisms, whether archaeal, bacterial, or both. The chapters in this book are divided into four sections. Section One covers methods that enable the study of the structure of the bacterial/archaeal chromosome, the main template for all gene regulatory processes, and its epigenetic modification. Section Two looks at a selection of approaches that enable higher levels of understanding of transcription initiation, a key step in information processing. Section Three discusses the investigation of regulating transcription factors, which are often considered the main players in gene regulation in prokaryotic cells. The Fourth Section focuses on the next stage of information processing at which gene regulation occurs, namely the RNA-based level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Prokaryotic Gene Regulation: Methods and Protocols is a valuable resource for researchers interested in learning more about this diverse field.
Автор: Hacker Название: Recombinant Protein Expression in Mammalian Cells ISBN: 1493987291 ISBN-13(EAN): 9781493987290 Издательство: Springer Рейтинг: Цена: 14025.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.
Автор: Brigitte Gasser; Diethard Mattanovich Название: Recombinant Protein Production in Yeast ISBN: 1493990233 ISBN-13(EAN): 9781493990238 Издательство: Springer Рейтинг: Цена: 15855.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.
Описание: In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
Название: Methods in recombinant protein production ISBN: 0128211776 ISBN-13(EAN): 9780128211779 Издательство: Elsevier Science Рейтинг: Цена: 23412.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Calm for your Soul: 365 Days of Spiritual Tranquillity is a collection of spiritual and inspiring writing providing hope and comfort for every single day of the year. Each page begins with a statement followed by an encouraging thought. Relevant scriptural passages are included for readers who desire a deeper time of reflection.
Описание: This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science.Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification.In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.
Автор: W.N. Drohan; Leon W. Hoyer Название: Recombinant Technology in Hemostasis and Thrombosis ISBN: 1461366445 ISBN-13(EAN): 9781461366447 Издательство: Springer Рейтинг: Цена: 6097.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The speakers described the essential features of the genes responsible for key plasma proteins important in hemostasis, including procoagulant Factors VIII and IX and anticoagulant proteins, Antithrombin III and Protein C.
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