Описание: This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.
Описание: This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system. The contents explore methods for cloning and expression of membrane proteins and membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Expression, Purification, and Structure Biology of Membrane Proteins serves to guide and encourage young researchers and newcomers to the field to tackle bold new studies on membrane proteins. Chapter 11 is available open access under a CC-BY 4.0 license via link.springer.com.
Автор: Gerhard Isenberg Название: Cytoskeleton Proteins ISBN: 3642489613 ISBN-13(EAN): 9783642489617 Издательство: Springer Рейтинг: Цена: 6097.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The standard protocols for the purification of all known cytoskeleton proteins are presented in this manual. The isolation procedure for each protein is shown in a clear flowchart, while the source of the protein, equipment and material needed, a list of suppliers, standard references, accession No.
Membrane Proteins - Engineering, Purification and Crystallization, a volume of Methods In Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol for the interested audience. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.
Автор: Bhupendra Pushkar Название: Protein Purification ISBN: 1774077027 ISBN-13(EAN): 9781774077023 Издательство: Mare Nostrum (Eurospan) Рейтинг: Цена: 22730.00 р. Наличие на складе: Поставка под заказ.
Описание: Describes the concepts underlying the process of protein purification. The strategies and equipment needed in protein purification is described. Advanced techniques like Polyacrylamide Gel Electrophoresis and Mass spectroscopy are also explained.
Автор: Argelia Lorence Название: Recombinant Gene Expression ISBN: 1493962213 ISBN-13(EAN): 9781493962211 Издательство: Springer Рейтинг: Цена: 20123.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Recombinant gene expression research has brought new advances such as the birth of the `omics` technologies. Here, expert researchers detail many RGE methods now used, including those for bacteria, fungi, plants and plant cells, and animals and animal cells.
Автор: Hacker Название: Recombinant Protein Expression in Mammalian Cells ISBN: 1493987291 ISBN-13(EAN): 9781493987290 Издательство: Springer Рейтинг: Цена: 14025.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.
Автор: Jacqueline MacDonald; Igor Kolotilin; Rima Menassa Название: Recombinant Proteins from Plants ISBN: 1493932888 ISBN-13(EAN): 9781493932887 Издательство: Springer Рейтинг: Цена: 11586.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This volume provides up-to-date scientific achievements from the world's top researchers. RecombinantProteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, RecombinantProteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.
Автор: House, Patrick Название: Recombinant Proteins in Plants ISBN: 1071622404 ISBN-13(EAN): 9781071622407 Издательство: Springer Рейтинг: Цена: 15855.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression.
Автор: G. Subramanian Название: Antibodies ISBN: 1461347017 ISBN-13(EAN): 9781461347019 Издательство: Springer Рейтинг: Цена: 18294.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
If the antibody industry is to achieve its full potential in the next decade, the individual technical potentials must be exploited, the limitations must be addressed, and lessons learned must be applied both to current purification methods and to the new technologies that continue to emerge. This book presents an overview of the current advances applied in the manufacture of monoclonal antibody including: -concepts in development of manufacturing strategies, -importance of antibody fragments, -application of chromatography method development, -quality control, -effect of expression on antibody properties, -virus removal and safety, -pharmacokinetics, -regulatory aspects.
Antibodies Volume 1: Production and Purification together with Volume2: Novel Technologies and Therapeutic Use will be of great value to all of those who are actively working in the field of antibodies. Volume 2: Novel Technologies and Therapeutic Use brings to the forefront current advances in novel technologies for the manufacturing of monoclonal antibodies and also their extensive clinical importance. Following on from Antibodies Volume 1: Production and Purification, this volume concentrates on: -new technologies in the manufacture of monoclonal antibodies, -the application of antibodies in cancer therapy and functional genomic therapy, -the importance of antibodies and prospects of antibody engineering for the future.
Автор: Robert K. Scopes Название: Protein Purification ISBN: 0387940723 ISBN-13(EAN): 9780387940724 Издательство: Springer Рейтинг: Цена: 13677.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: New textbooks at all levels of chemistry appear with great regularity. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc- tive introductions to their fields.
Автор: Simpson Richard J., Adams Peter D., Golemis Erica Название: Basic Methods in Protein Purification and Analysis: A Laboratory Manual ISBN: 0879698675 ISBN-13(EAN): 9780879698676 Издательство: Неизвестно Рейтинг: Цена: 13380.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing proteinDSprotein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods. The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and ProteinDSProtein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts--a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing proteinDSprotein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases.
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