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Heterologous Expression of Membrane Proteins, Mus-Veteau


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Цена: 18904.00р.
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Автор: Mus-Veteau
Название:  Heterologous Expression of Membrane Proteins
ISBN: 9781071623671
Издательство: Springer
Классификация:

ISBN-10: 1071623672
Обложка/Формат: Hardback
Страницы: 467
Вес: 1.11 кг.
Дата издания: 15.07.2022
Серия: Methods in Molecular Biology
Язык: English
Издание: 3rd ed. 2022
Иллюстрации: 76 tables, color; 78 illustrations, color; 16 illustrations, black and white; xvii, 467 p. 94 illus., 78 illus. in color.
Размер: 254 x 178
Читательская аудитория: Professional & vocational
Основная тема: Life Sciences
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Описание: This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.
Дополнительное описание: Solubilization and Purification of a5b1 Integrin from Rat Liver for Reconstitution into Nanodiscs.- Membrane Proteins Production in Escherichia coli: Protocols and Rules.- Functional Overexpression of Membrane Proteins in E. coli: The Good, the Bad, and t



Heterologous Expression of Membrane Proteins

Автор: Mus-Veteau
Название: Heterologous Expression of Membrane Proteins
ISBN: 1493936352 ISBN-13(EAN): 9781493936359
Издательство: Springer
Рейтинг:
Цена: 17074.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs). Membraneproteins play a key role in numerous pathologies such as cancer, cysticfibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies onthese and other disorders are hampered by a lack of information about theproteins involved. This book sets out to aid researchers in rectifying thissituation. Written for the highly successful Methods in Molecular Biology series, chapters include introductionsto their respective topics, lists of the necessary materials and reagents,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritative and up-to-date, HeterologousExpression of Membrane Proteins: Methods and Protocols, Second Editionserves as an ideal guide for scientists attempting to delve deeper into the myriadunique IMP structures.

Heterologous Expression of Membrane Proteins

Автор: Isabelle Mus-Veteau
Название: Heterologous Expression of Membrane Proteins
ISBN: 1493981021 ISBN-13(EAN): 9781493981021
Издательство: Springer
Рейтинг:
Цена: 12196.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs).

Expression, Purification, and Structural Biology of Membrane Proteins

Автор: Camilo Perez; Timm Maier
Название: Expression, Purification, and Structural Biology of Membrane Proteins
ISBN: 1071603728 ISBN-13(EAN): 9781071603727
Издательство: Springer
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Цена: 15855.00 р.
Наличие на складе: Нет в наличии.

Описание: This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system. The contents explore methods for cloning and expression of membrane proteins and membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Expression, Purification, and Structure Biology of Membrane Proteins serves to guide and encourage young researchers and newcomers to the field to tackle bold new studies on membrane proteins. Chapter 11 is available open access under a CC-BY 4.0 license via link.springer.com.


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