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RNA Modifications: Methods and Protocols, McMahon Mary


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Цена: 14635.00р.
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Автор: McMahon Mary
Название:  RNA Modifications: Methods and Protocols
ISBN: 9781071613733
Издательство: Springer
Классификация:
ISBN-10: 1071613731
Обложка/Формат: Hardcover
Страницы: 427
Вес: 0.95 кг.
Дата издания: 05.07.2021
Язык: English
Размер: 25.91 x 19.56 x 2.54 cm
Ссылка на Издательство: Link
Поставляется из: Германии
Описание: This detailed book aims to provide an overview of novel bioinformatics resources for exploring diverse aspects of RNA biology. This edition focuses on methods dealing with non-coding RNA (miRNAs, circRNAs or lncRNAs), RNA modifications (m6A or RNA editing), single cell RNA-seq and statistical models to handle count data from RNA-seq experiments.


Matrix Metalloproteinase Protocols

Автор: Ian M. Clark
Название: Matrix Metalloproteinase Protocols
ISBN: 1603272984 ISBN-13(EAN): 9781603272988
Издательство: Springer
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Цена: 12806.00 р. 18294.00 -30%
Наличие на складе: Есть (1 шт.)
Описание: Detailing proven laboratory techniques for the study of MMPs, this book covers the methods including those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a variety of circumstances.

RNA-Protein Complexes and Interactions

Автор: Ren-Jang Lin
Название: RNA-Protein Complexes and Interactions
ISBN: 1493935895 ISBN-13(EAN): 9781493935895
Издательство: Springer
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Цена: 17074.00 р.
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Описание:

1. Characterization of RNA-Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2

Lindsey Skrdlant and Ren-Jang Lin

2. Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification)

Je-Hyun Yoon and Myriam Gorospe

3. Biotin-Streptavidin Affinity Purification of RNA-Protein Complexes Assembled In Vitro

Shuai Hou, Lei Shi, and Haixin Lei

4. Detecting RNA-Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting

Xuexiu Zheng, Sunghee Cho, Heegyum Moon, Tiing Jen Loh, Hana Jang, and Haihong Shen

5. Purification of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture

Naoyuki Kataoka

6. Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts

Keith T. Gagnon

7. In Vitro Analysis of Ribonucleoprotein Complex Remodeling and Disassembly

Hua-Lin Zhou and Hua Lou

8. Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases

Ya-Ming Hou

9. Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles

Chao Huang, Guowei Wu, and Yi-Tao Yu

10. Northwestern Blot Analysis: Detecting RNA-Protein Interaction After Gel Separation of Protein Mixture

Shangbing Zang and Ren-Jang Lin

11. Measuring mRNA Translation by Polysome Profiling

Marek Kudla and Fedor V. Karginov

12. Genome-Wide Profiling of RNA-Protein Interactions Using CLIP-Seq

Cheryl Stork and Sika Zheng

13. Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichment

Sarah F. Mitchell and Roy Parker

14. Ribo-Proteomics Approach to Profile RNA-Protein and Protein-Protein Interaction Networks

Hsin-Sung Yeh, Jae-Woong Chang, and Jeongsik Yong

15. Detection of Protein-Protein Interaction Within an RNA-Protein Complex Via Unnatural-Amino-Acid-Mediated Photochemical Crosslinking

Fu-Lung Yeh, Luh Tung, and Tien-Hsien Chang

16. Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX

Jiehua Zhou and John J. Rossi

17. mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA Binding Proteins

Sebastien M. Weyn-Vanhentenryck and Chaolin Zhang

18. Design

RNA Remodeling Proteins

Автор: Marc Boudvillain
Название: RNA Remodeling Proteins
ISBN: 1493922130 ISBN-13(EAN): 9781493922130
Издательство: Springer
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Цена: 18294.00 р.
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Recombinant and In Vitro RNA Synthesis

Автор: Graeme L. Conn
Название: Recombinant and In Vitro RNA Synthesis
ISBN: 1493958305 ISBN-13(EAN): 9781493958306
Издательство: Springer
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Цена: 14635.00 р.
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Описание: Part of Springer`s Methods in Molecular Biology series, this collection of detailed protocols guides the reader from the start of a new RNA project to the final sample, with step-by-step protocols and expert tips on deploying catalytic RNAs in the laboratory.

RNA Modifications

Автор: McMahon
Название: RNA Modifications
ISBN: 1071613766 ISBN-13(EAN): 9781071613764
Издательство: Springer
Рейтинг:
Цена: 14635.00 р.
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Описание: This detailed book describes some of the most recent advances and up-to-date methodologies to detect, quantify, analyze, and elucidate the biological function of different types of RNA modifications. Importantly, the methodologies and tools described herein can be applied to a wide variety of organisms and can be used to address biological and clinical questions. Beginning with a section on bioinformatics tools, the collection continues with sections on detecting RNA modifications using Nanopore direct RNA sequencing, next-generation sequencing approaches, qPCR- and molecular biology-based methods, mass spectrometry- and NMR-based methods, as well as approaches to assess kinetics, determinants, and functions of RNA modifications. Written for the highly successful Methods in Molecular Biology series style, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Modifications: Methods and Protocols serves as an ideal guide for those working directly in the fields of epitranscriptomics and post-transcriptional gene regulation, as well as for scientists and clinicians interested in bioinformatic tools to study RNA modifications and techniques to dissect their roles in physiology and disease. Chapter 20 is available open access under a CC BY 4.0 license.

Protein Bioinformatics

Автор: Cathy H. Wu; Cecilia N. Arighi; Karen E. Ross
Название: Protein Bioinformatics
ISBN: 1493967819 ISBN-13(EAN): 9781493967810
Издательство: Springer
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Цена: 18294.00 р.
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Описание: This volume introduces bioinformatics research methods for proteins, with special focus on protein post-translational modifications (PTMs) and networks.

Post-translational Modifications of Proteins

Автор: Christoph Kannicht
Название: Post-translational Modifications of Proteins
ISBN: 1617377392 ISBN-13(EAN): 9781617377396
Издательство: Springer
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Цена: 17684.00 р.
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Описание: This is a fully up-dated and expanded practical guide to protein structure-function relationships. The book is the most comprehensive volume on PTM analysis available. This important area of research is brought up-to-date by the leading scientists in the field.

Peptide Modifications to Increase Metabolic Stability and Activity

Автор: Predrag Cudic
Название: Peptide Modifications to Increase Metabolic Stability and Activity
ISBN: 1627036512 ISBN-13(EAN): 9781627036511
Издательство: Springer
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Цена: 12805.00 р.
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Описание: This book provides summarized methods for the preparation, purification of modified peptides, and assessment of their biochemical activities. It features step-by-step detail essential for reproducible results and expert implementation advice.

Post-Translational Modifications That Modulate Enzyme Activity,626

Автор: Garcia, Benjamin A.
Название: Post-Translational Modifications That Modulate Enzyme Activity,626
ISBN: 0128186690 ISBN-13(EAN): 9780128186695
Издательство: Elsevier Science
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Цена: 26275.00 р.
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Описание:

Post-translational Modifications That Modulate Enzyme Activity, Volume 626 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Updated chapters include Crosstalk between cellular metabolism and histone acetylation, Isolation of protein complexes and modifications that regulate transcriptional machinery, High-throughput phosphoproteome mapping through multiplexed mass spectrometry, Differentiation of D and L epimerization in proteins, Biochemical analysis of protein arginylation, Site-specific Determination of lysine acetylation stoichiometries on the proteome-scale, Genomic and biochemical analysis of RNA post-transcriptional modifications, Isolation and characterization of glycosylated (neuro)peptides, and more.

Synthetic And Enzymatic Modifications Of The Peptide Backbone,655

Автор: Petersson, E.James
Название: Synthetic And Enzymatic Modifications Of The Peptide Backbone,655
ISBN: 0128212535 ISBN-13(EAN): 9780128212530
Издательство: Elsevier Science
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Цена: 26275.00 р.
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Описание:

Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

Modifications And Targeting Of Protein Termini Part A,684

Автор: Arnesen,Thomas
Название: Modifications And Targeting Of Protein Termini Part A,684
ISBN: 0443157723 ISBN-13(EAN): 9780443157721
Издательство: Elsevier Science
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Цена: 23244.00 р.
Наличие на складе: Нет в наличии.

Analysis of Post-Translational Modifications and Proteolysis in Neuroscience

Автор: Jennifer Elizabeth Grant; Hong Li
Название: Analysis of Post-Translational Modifications and Proteolysis in Neuroscience
ISBN: 1493980580 ISBN-13(EAN): 9781493980581
Издательство: Springer
Рейтинг:
Цена: 12196.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание:

1. Proteomic Analysis of Post-Translational Modifications in Neurobiology

Hongbo Gu, Matthew P. Stokes, and Jeffrey C. Silva

2. A Boronic Acid-based Enrichment for Site-specific Identification of the N-glycoproteome using MS-based Proteomics

Haopeng Xiao, George X. Tang, Weixuan Chen, and Ronghu Wu

3. Site-specific Localization of D-Amino Acids in Bioactive Peptides by Ion Mobility Spectrometry

Chenxi Jia, Christopher B. Lietz, Qing Yu, and Lingjun Li

4. Quantitative Profiling of Reversible Cysteome Modification under Nitrosative Stress

Yue-Ting Wang, Sujeewa C. Piyankarage, and Gregory R. J. Thatcher

5. Label-free LC-MS/MS Comparative Analysis of Protein S-nitrosome in Synaptosomes from Wild Type and APP Transgenic Mice

Monika Zaręba-Koziol, Maciej Lalowski, and Aleksandra Wyslouch-Cieszyńska

6. Proteomics Identification of Redox-Sensitive Nuclear Protein Targets Of Human Thioredoxin 1 from SHSY-5Y Neuroblastoma Cell Line

Mohit Raja Jain, Changgong Wu, Qing Li, Chuanlong Cui, Huacheng Dai, and Hong Li

7. Identification and Quantification of K63-Ubiquitinated Proteins in Neuronal Cells by Hi

gh-

Resolution Mass Spectrometry

Gustavo Monteiro Silva, Wei Wei, Sandhya Manohar, and Christine Vogel

8. Phosphorylation Site Profiling of NG108 Cells Using Quadrupole/Orbitrap Mass

Spectrometry

Fang-Ke Huang, Guoan Zhang, and Thomas A. Neubert

9. Phosphoproteomics of Tyrosine Kinases in the Nervous System

Robert J. Chalkley and

Ralph A. Bradshaw

10. Analysis of PINK1 and CaMKII Substrates Using Mass Spectrometry-Based Proteomics

Yan Li, Lesley A. Kane, Michael A Bemben, and Katherine W Roche

11. Liquid Chromatography-Tandem Mass Spectrometry Analysis of Tau Phosphorylation

Jhoana Mendoza, Georgia Dolios, and Rong Wang

12. Identification of Protease Substrates in Complex Proteomes by iTRAQ-TAILS

Tobias Kockmann, Nathalie Carte, Samu Melkko, and Ulrich auf dem Keller

13. Proteolytic P

rocessing of Neuropeptides

Lloyd D. Fricker

14 Isotope Dilution Analysis of Myelin Basic Protein Degradation after Brain Injury

Andrew K Ottens

15. Identification and Characterization of Protein Post-Translational Modifications by

Differential Fluorescent Labeling

Eric D. Hamlett, Cristina Osorio, and Oscar Alzate

16 Mass

Spectrometric Detection of Detyrosination and Polyglutamyation on the C-Terminal Region of Brain Tubulin

Yasuko Mori, Alu Konno, Mitsutoshi Setou, and Koji Ikegami

17. Determination of polyhydroxybutyrate (PHB) Post-Translational Modifications of Proteins Using Mass Spectrometry

Tong Liu, Wei Chen, Stacey Pan, Chuanlong Cui, Hong Li, and Eleonora Zakharian


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