Plant Transcription Factors: Methods and Protocols, Yuan Ling, Perry Sharyn E.
Автор: Lutz Nover Название: Plant Promoters and Transcription Factors ISBN: 366222304X ISBN-13(EAN): 9783662223048 Издательство: Springer Рейтинг: Цена: 6097.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The control of plant gene expression at the transcriptional level is the main subject of this volume. Formation of specific DNA-protein complexes - activating or repressing transcription - is the crux of developmental or environmental control of gene expression.
Автор: Yamaguchi Название: Plant Transcription Factors ISBN: 1493986562 ISBN-13(EAN): 9781493986569 Издательство: Springer Рейтинг: Цена: 15855.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed book provides general protocols and technologies that plant biologists worldwide often utilize for the purpose of accelerating research progress in the field of plant transcription factors.
Описание: The genome is more than a linear code as depicted by its DNA sequences as several interacting factors play a crucial role in shaping its organization and function. The complete sequences of a number of plant genomes and the recent advances of high-throughput technologies has fueled research efforts in the field of Plant Nuclear Biology unveiling numerous insights about the mechanisms underlying genome regulation. Genomic information is being integrated into molecular- and cellular-level mechanisms of the plant processes. A host of nuclear processes underlie key developmental processes as well as biotic and abiotic interactions. Non-coding RNAs have been increasingly recognized as players in gene expression and genome defense and integrity. However, in vivo, genomes exist as elaborate physical structures, and their functional properties are strongly determined by their cellular organization. Various types of subcellular structure have been identified in the nucleus, which are associated with transcription factors, RNA processing proteins and epigenetic regulators. Interestingly, these nuclear bodies display different behaviors in response to the environment. This book compiles a series of landmark discussions of the recent advances in plant nuclear biology research focusing in the functional relevance of the arrangement of genomes and nuclear processes that impact plant physiology and development.
Описание: The complete sequences of a number of plant genomes and the recent advances of high-throughput technologies has fueled research efforts in the field of Plant Nuclear Biology unveiling numerous insights about the mechanisms underlying genome regulation.
Автор: Witold Filipowicz; Thomas Hohn Название: Post-Transcriptional Control of Gene Expression in Plants ISBN: 9401066442 ISBN-13(EAN): 9789401066440 Издательство: Springer Рейтинг: Цена: 13415.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Palme, ed.) Plant Molecular Biology 26, 1237-1679) described the relay races by which signals are transported in plants from the sites of stimuli to the gene expression machinery of the cell.
Автор: Shan Libo, He Ping Название: Plant Pattern Recognition Receptors: Methods and Protocols ISBN: 1493983202 ISBN-13(EAN): 9781493983209 Издательство: Springer Рейтинг: Цена: 18294.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools.
Автор: Dissmeyer Nico, Schnittger Arp Название: Plant Kinases: Methods and Protocols ISBN: 149395721X ISBN-13(EAN): 9781493957217 Издательство: Springer Рейтинг: Цена: 14635.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
Автор: Kianian Shahryar F., Kianian Penny M. Avoles Название: Plant Cytogenetics: Methods and Protocols ISBN: 1493980971 ISBN-13(EAN): 9781493980970 Издательство: Springer Рейтинг: Цена: 15902.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume covers arange of methods used in plant cytogenetics, beginning with basic analysis ofchromosomes and visualizing gene locations, to manipulating and dissectingchromosomes, and then focusing on less understood features of chromosomes suchas recombination initiation sites and epigenomic marks.
Описание: This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
Описание: 1. Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal Ganeshan Sivanandhan, Natesan Selvaraj, Andy Ganapathi, and Markandan Manickavasagan 2. Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction Thanise Nogueira, Fьller, Jъlio Cйsar de Lima, Fernanco de Costa, Kelly C.S. Rodrigues-Corrкa, and Arthur G. Fett-Neto 3. A Modified Protocol for High Quality RNA Extraction from Oleoresin-Producing Adult Pines Jъlio Cйsar de Lima, Thanise Nogueira Fьller, Fernanda de Costa, Kelly C.S. Rodrigues-Corrкa, and Arthur G. Fett-Neto 4. Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier 5. From Plant Extract to a cDNA-Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica Fernando de Costa, Carla J.S. Barber, Darwin W. Reed, and Patrick S. Covello 6. Production of Recombinant Caffeine Synthase from Guarana (Paullinia cupana var. sorbillis) in Escherichia coli Flбvia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera 7. Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala Vishal Singh Negi and Dulal Borthakur 8. Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast Anita Emmerstorfer and Harald Pichler 9. Purification of a Recombinant Polyhistidine-Tagged Glycosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC) Fernanda de Costa, Carla J.S. Barber, Pareshkumar T. Pujara, Darwin W. Reed, and Patrick S. Covello 10. A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes Morten Egevang Jrgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier 11. Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters Mбrcia R. de Almeida and Martina V. Strцmvik
12. Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants Inкs Carqueijeiro, Viviana Martins, Henrique Noronha, Hernвni Gerуs, and Mariana Sottomayor 13. Protoplast Transformation as a Plant-Transferable Transient Expression System Patrнcia Duarte, Diana Ribeiro, Inкs Carqueijeiro, Sara Bettencourt, and Mariana Sottomayor
14. Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System Basdeo Bhagwat, Ming Chi, Dianwei Han, Haifeng Tang, Guiliang Tang, and Yu Xiang
15. Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs Ming Chi, Basdeo Bhagwat, Guiliang Tang, and Yu Xiang
Автор: Aalen Reidunn Birgitta Название: Plant Receptor Kinases: Methods and Protocols ISBN: 1493983822 ISBN-13(EAN): 9781493983827 Издательство: Springer Рейтинг: Цена: 15855.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways.
Автор: Busch Wolfgang Название: Plant Genomics: Methods and Protocols ISBN: 1493983644 ISBN-13(EAN): 9781493983643 Издательство: Springer Рейтинг: Цена: 17074.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Part I: Genotypes
1. CRISPR/Cas-mediated in planta Gene Targeting
Simon Schiml, Friedrich Fauser, and Holger Puchta
2. User Guide for the LORE1 Insertion Mutant Resource
Terry Mun, Anna Malolepszy, Niels Sandal, Jens Stougaard, and Stig U. Andersen
3. Enabling Reverse Genetics in Medicago truncatula Using High Throughput Sequencing for Tnt1 Flanking Sequence Recovery
Xiaofei Cheng, Nick Krom, Shulan Zhang, Kirankumar S. Mysore, Michael Udvardi, and Jiangqi Wen
4. Generation of Doubled Haploid Lines for QTL Mapping
Daniele L. Filiault, Danelle K. Seymour, Ravi Maruthachalam, and Julin N. Maloof
Part II: Phenotypes
5. Assessing Distribution and Variation of Genome-wide DNA Methylation using Short Read Sequencing
Jцrg Hagmann and Claude Becker
6.Circular Chromosome Conformation Capture in Plants
Stefan Grob
7. Genome Wide Profiling of Histone Modifications and Histone Variants in Arabidopsis thaliana and Marchantia polymorpha
8. Tissue-Specific Transcriptome Profiling in Arabidopsis Roots
Erin E. Sparks and Philip N. Benfey
9. Sample Preparation Protocols for Protein Abundance, Acetylome and Phosphoproteome Profiling of Plant Tissues
Gaoyuan Song, Maxwell R McReynolds, and Justin W Walley
10. Automated High-throughput Root Phenotyping of Arabidopsis thaliana under Nutrient Deficiency Conditions
Santosh B. Satbhai, Christian Göschl, and Wolfgang Busch
11. Large-scale Phenotyping of Root Traits in the Model Legume Lotus japonicus
Marco Giovannetti, Anna Malolepszy, Christian Goeschl, and Wolfgang Busch
12. Long-term Confocal Imaging of Arabidopsis thaliana Roots for Simultaneous Quantification of Root Growth and Fluorescent Signals
Delyana Stoeva, Christian Gцschl, and Wolfgang Busch
Part III: Molecular Bases of Phenotypes
13. Identification of Protein-DNA Interactions Using Enhanced Yeast One-Hybrid Assays and a Semi-Automated Approach
Allison Gaudinier, Michelle Tang, Anne-Maarit Bеgman, and Siobhan M. Brady
14. Mapping Protein- protein Interaction using High-throughput Yeast-2-Hybrid
Jessica Lopez and M. Shahid Mukhtar
15.Mapping Protein-protein Interactions using Affinity Purification and Mass Spectrometry
Chin-Mei Lee, Christopher Adamchek, Ann Feke, Dmitri A. Nusinow, and Joshua M. Gendron
16. Measuring Protein Movement, Oligomerization State, and Protein-Protein Interaction in Arabidopsis Roots Using Scanning Fluorescence Correlation Spectroscopy (scanning FCS) Natalie M. Clark and Rosangela Sozzani
17.Studying Protein-Protein Interactions in planta using Advanced Fluorescence Microscopy
Marc Somssich and Rьdiger Simon
18. Chemiluminescence Based Detection of Peptide Activity and Peptide-Receptor Binding in Plants
Mari Wildhagen, Markus Albert, and Melinka A. Butenko
19. Application of Chemical Genomics to Plant-Bacteria Communication: A High-Throughput System to Identify Novel Molecules Modulating the Induction of Bacterial Virulence Genes by Plant Signals
Elodie Vandelle, Maria Rita Puttilli, Andrea Chini, Giulia Devescovi, Vittorio Venturi, and Annalisa Polverari
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